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• Glutathione S-transferase (GST): • Catalyzes conjugations of the substrate glutathione (GSH) • GST is an enzyme and binds GSH in an enzyme-substrate complex • Enzyme (E) + Substrate (S)  Enzyme-substrate complex • Interaction is dynamic and GST will bind reversibly Substrate Enzyme Enzyme-substrate complex GST GSH BY1101 Introduction to Molecular and Cellular Biology Affinity chromatography What is thesource of the GST

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